Pre-Filter Cleaning of Laminar Air Flow (LAF) :.Note: Replace the UV Light after 999 Burning hrs. Enter the burning hour details of UV in Annexure-2. After 30 minutes switch OFF the UV and switch ON the Visible light.Turn the switch of the UV lights to ON position.Ensure that the differential pressure is within the limit of 8 to 15 mm of water and record the observation in Annexure-2. Turn the switch of the Air Flow to ON position.Ensure that the manometer is showing Zero reading before starting.Laminar Air Flow Operation (LAF-Operation).Finally Routine culture handling shall perform at the end of the Sample testing only.After that spray 70% IPA and switch off the visible light and then switch ON the UV light till to start the next operation.Then clean properly the working chamber from ceiling, followed by side glass and then the platform of the chamber with 70% IPA.First of all, Turn OFF the switch of the Air Flow and UV of LAF unit (if ON).LAF: Laminar Air Flow: Airflow which is linear and positive up to working surfaces and thus prevents contamination of surrounding viable/ non viable particulate matter in aseptic handling.Standard Operating Procedure (SOP): A written authorized procedure, which gives instructions for performing operations. ![]() This Standard Operating Procedure is applicable at Microbiology section / Sterile Area where Laminar Air Flow (LAF) installed.To lay down the procedure for the operation, cleaning, maintenance and qualification of the Laminar Air Flow.Laminar Air flow (LAF) – Operation, Cleaning and Qualification Purpose: Note: Replace the UV Light after 999 Burning hrs.Laminar Air flow (LAF) – Operation, Cleaning and Qualification.
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